Two Postdoctoral Research Positions Available in Truman Lab Starting Spring 2021!

Understanding the Interplay between Hsp70 and the DNA damage response in mammalian cells 

 

Molecular chaperones stabilize many important proteins in signal transduction. Over the past few years, evidence has accumulated that Hsp70 is critical for the response to DNA damage. The candidate will study the interactions between Hsp70 and proteins in the AMT/ATR pathways in mammalian cells and novel ways to exploit these interactions in cancer cells. The project will involve a range of molecular technologies including mass spectrometry, CRISPR-genome engineering and high-throughput screening. 

Required qualifications:

  • Ph.D in biochemistry, molecular biology, cancer biology or related field.

  • Experience with mammalian cell culture including passaging of cells, transfection etc.

  • Experience with general molecular techniques such as Western Blotting, DNA cloning, co-immunoprecipitation

  • Background in signal transduction/DNA damage response/ATM/ATR pathways 

NIH Funding is available for 4 years, with the expectation that the candidate will work with Dr. Truman to apply for fellowships during this time. Ideal candidates will have graduated less than 1 year from their start date in the Truman lab.

 

To apply, please see:  https://jobs.uncc.edu/postings/33501 

Understanding the Chaperone Code in yeast

 

Although chaperones have been studied for over 50 years, little is known about the numerous post-translational modifications that impact in vitro and in vivo chaperone function (the "chaperone code"). These modifications likely come from kinases activated in diverse processes such as the DNA damage response, autophagy, MAP kinase signaling and related processes.

 

The candidate will study the role of the Hsp70 and Hsp40 chaperone code primarily in yeast, but also in mammalian cells. The project will involve a range of molecular technologies including mass spectrometry, site-directed mutagenesis and high-throughput screening. 

Required qualifications:

  • Ph.D in biochemistry, molecular biology, cancer biology or related field.

  • Experience with yeast (saccharomyces cerevisiae) including transformation, gene deletion, epitope tagging of genes

  • Experience with general molecular techniques such as Western Blotting, DNA cloning, co-immunoprecipitation

  • Background in chaperones (in vitro or yeast assays) 

  • Expertise in yeast signal transduction

NIH Funding is available for 4 years, with the expectation that the candidate will work with Dr. Truman to apply for fellowships during this time. Ideal candidates will have graduated less than 1 year from their start date in the Truman lab.

 

To apply please see: https://jobs.uncc.edu/postings/33500

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